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mouse anti human psgl 1 blocking antibody  (Bio-Techne corporation)


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    Bio-Techne corporation mouse anti human psgl 1 blocking antibody
    Mouse Anti Human Psgl 1 Blocking Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+psgl+1+blocking+antibody/Mouse+anti-Human+IgG+Fc+Secondary+Antibody+(MK+1+A6)/pm38243063-236-15-22
    Average 90 stars, based on 7 article reviews
    mouse anti human psgl 1 blocking antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Blocking Assay:

    Article Title: PSGL-1 decorated with sialyl Lewis a/x promotes high affinity binding of myeloma cells to P-selectin but is dispensable for E-selectin engagement.
    Article Snippet: .. In some experiments, cells were pre-incubated for 1 h before the rolling assay with a mouse anti-human PSGL-1 blocking antibody (clone 688,102; Bio-techne) or mouse isotype control (clone 11711; Bio-techne) at 10 μg/ml or pre-treated/mock-treated with neuraminidase from Vibrio cholerae (1 mU/ml) or pronase (1 mg/ ml) for 45 min at RT in serum free RPMI1640 media. .. Cells (5 × 105/tube) were collected, washed in DPBS, resuspended in 100 μl stained buffer, stained with PEconjugated anti-human cutaneous lymphocyte antigen antibody clone Heca452, Alexa-fluor 647-conjugated anti-human PSGL-1 clone KPL-1 (all from BD Biosciences) and incubated for 30 min at RT with gentle rocking.

    Article Title: PSGL-1 decorated with sialyl Lewis a/x promotes high affinity binding of myeloma cells to P-selectin but is dispensable for E-selectin engagement
    Article Snippet: .. In some experiments, cells were pre-incubated for 1 h before the rolling assay with a mouse anti-human PSGL-1 blocking antibody (clone 688,102; Bio-techne) or mouse isotype control (clone 11711; Bio-techne) at 10 μg/ml or pre-treated/mock-treated with neuraminidase from Vibrio cholerae (1 mU/ml) or pronase (1 mg/ml) for 45 min at RT in serum free RPMI1640 media. .. Cells (5 × 10 5 /tube) were collected, washed in DPBS, resuspended in 100 μl stained buffer, stained with PE-conjugated anti-human cutaneous lymphocyte antigen antibody clone Heca452, Alexa-fluor 647-conjugated anti-human PSGL-1 clone KPL-1 (all from BD Biosciences) and incubated for 30 min at RT with gentle rocking.

    Control:

    Article Title: PSGL-1 decorated with sialyl Lewis a/x promotes high affinity binding of myeloma cells to P-selectin but is dispensable for E-selectin engagement.
    Article Snippet: .. In some experiments, cells were pre-incubated for 1 h before the rolling assay with a mouse anti-human PSGL-1 blocking antibody (clone 688,102; Bio-techne) or mouse isotype control (clone 11711; Bio-techne) at 10 μg/ml or pre-treated/mock-treated with neuraminidase from Vibrio cholerae (1 mU/ml) or pronase (1 mg/ ml) for 45 min at RT in serum free RPMI1640 media. .. Cells (5 × 105/tube) were collected, washed in DPBS, resuspended in 100 μl stained buffer, stained with PEconjugated anti-human cutaneous lymphocyte antigen antibody clone Heca452, Alexa-fluor 647-conjugated anti-human PSGL-1 clone KPL-1 (all from BD Biosciences) and incubated for 30 min at RT with gentle rocking.

    Article Title: PSGL-1 decorated with sialyl Lewis a/x promotes high affinity binding of myeloma cells to P-selectin but is dispensable for E-selectin engagement
    Article Snippet: .. In some experiments, cells were pre-incubated for 1 h before the rolling assay with a mouse anti-human PSGL-1 blocking antibody (clone 688,102; Bio-techne) or mouse isotype control (clone 11711; Bio-techne) at 10 μg/ml or pre-treated/mock-treated with neuraminidase from Vibrio cholerae (1 mU/ml) or pronase (1 mg/ml) for 45 min at RT in serum free RPMI1640 media. .. Cells (5 × 10 5 /tube) were collected, washed in DPBS, resuspended in 100 μl stained buffer, stained with PE-conjugated anti-human cutaneous lymphocyte antigen antibody clone Heca452, Alexa-fluor 647-conjugated anti-human PSGL-1 clone KPL-1 (all from BD Biosciences) and incubated for 30 min at RT with gentle rocking.



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